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The Application of Quantification Genomics in the Development of Autogamous Plants with Chloroplast DNA Variability in Wild Brassicas and their Biology Research

Feroz Lu

In order to select inbred lines more effectively in light of the rising demand for food, it is necessary to employ techniques and alternatives. Particularly when the pedigree technique is applied to autogamous plants, quantitative genetics play a significant role in this regard. This study suggests using the best linear unbiased predictor (BLUP) in conjunction with relationship information between progenies to provide breeding values that are more accurate and, as a outcome boost genetic benefits via selection. A proposal is put forth to speed up the process of obtaining perennial plant inbreds and use as much data as possible during selection to ensure optimal accuracy. Inbreds that are superior to the ones already available might be made accessible more frequently in this way, helping the agricultural sector meet the demand for perennial plants.

In order to characterise the cytoplasm and conduct population genetics and phylogeographic analysis, it is crucial to assess the diversity of the chloroplast DNA (cpDNA) in wild relatives of crop brassicas. The former is helpful for breeding programmes that involve extensive hybridization and the synthesis of alloplasmic lines, whereas the latter is crucial for developing conservation methods. Consequently, the PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) technique was used to examine the cpDNA diversity in 14 wild brassicas, including 31 accessions, and the outcomes showed the presence of 219 polymorphic fragments in total. The combination of polymorphisms obtained by using only two primer pair-restriction enzyme combinations was sufficient to distinguish all 14 wild brassicas. Moreover, 11 primer pairs-restriction enzyme combinations revealed intraspecific polymorphisms in eight wild brassicas (including endemic and endangered species, B. cretica and B. insularis, resp.). Thus, even within a small number of accessions that were screened, intraspecific polymorphisms were observed, which is important for population genetics analyses in wild brassicas and consequently for conservation studies.