ஜர்னல் ஆஃப் அனலிட்டிகல் & பயோஅனாலிட்டிகல் டெக்னிக்ஸ்

சுருக்கம்

Simultaneous Estimation of Aceclofenac, Paracetamol and Tizanidine in Their Combined Dosage Forms by Spectrophotometric and RP- HPLC Method

Chandrashekhar Narajji, Hardik R Patel, Manohar D Karvekar and Suresh Babu AR

A rapid and sensitive RP-HPLC method with UV detection and UV spectrophotometric method for routine Pharmaceutical quality control of aceclofenac, paracetamol and tizanidine in tablets formulation was developed. Chromatography was performed by using Phenomenex-Luna C 18 (250 x 4.6 mm, 5μ) with a mobile phase containing Methanol: Water (90:10v/v), flow rate 1mL/min, wavelength at 256 nm. Linearity observed over the concentration range between 5-30μg/mL, 10-60μg/mL and 2-12μg/mL for aceclofenac, paracetamol and tizanidine respectively. The UV spectrophotometric method was performed at 277, 248 and 323 nm by derivative spectrophotometry with simultaneous equation method. The entire three drugs obey Beer’s law in the concentration range between 5-30 μg/mL, 2-16 μg/mL and 2-30 μg/mL respectively. The proposed methods were simple, rapid, precise, accurate and sensitive and can be used for routine quality control in pharmaceuticals.