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Low-Frequency, Whole Body Vibration Induced Neurite Outgrowth by Pc12m3 Cells with Impaired Nerve Growth Factor-Induced Neurite Outgrowth

Yoshihisa Koike, Reiko Tutida, Yuko Hayashi, Yoko Yamanishi and Yoshio Kano

Purpose: To investigate the effects of very low-frequency whole body vibration (WBV) stimulation using PC12 m3 cells.

Methods: Mutant, drug-hypersensitive, PC12 m3 cells were obtained by means of continuous culture of neural PC12 cells and then stimulated by low-frequency vibration at frequencies of 7–13 Hz for 5–20 min with simultaneous exposure to nerve growth factor (NGF).

Results: The frequency of neurite outgrowth by PC12 m3 cells induced by 7-Hz vibratory stimulation was approximately 3-fold greater than that induced by NGF alone. Moreover, activated cyclic-AMP responsive element (CRE)-binding protein (CREB) expression was induced in PC12 m3 cells stimulated by 7-Hz vibration.

Conclusion: Low-frequency vibratory stimulation induces neurite outgrowth via a p38 mitogen-activated protein kinase/CREB signaling pathway in PC12 m3 cells. Together, these results suggest that WBV is an efficient method to stimulate neurite growth.