எங்கள் குழு ஒவ்வொரு ஆண்டும் அமெரிக்கா, ஐரோப்பா மற்றும் ஆசியா முழுவதும் 1000 அறிவியல் சங்கங்களின் ஆதரவுடன் 3000+ உலகளாவிய மாநாட்டுத் தொடர் நிகழ்வுகளை ஏற்பாடு செய்து 700+ திறந்த அணுகல் இதழ்களை வெளியிடுகிறது, இதில் 50000 க்கும் மேற்பட்ட தலைசிறந்த ஆளுமைகள், புகழ்பெற்ற விஞ்ஞானிகள் ஆசிரியர் குழு உறுப்பினர்களாக உள்ளனர்.
அதிக வாசகர்கள் மற்றும் மேற்கோள்களைப் பெறும் திறந்த அணுகல் இதழ்கள்
700 இதழ்கள் மற்றும் 15,000,000 வாசகர்கள் ஒவ்வொரு பத்திரிகையும் 25,000+ வாசகர்களைப் பெறுகிறது
Christopher M Warner, Matthew S Croughan, George B and Joy Rathmann
From the point of DNA sequence confirmation until production of meaningful clinical quantities of novel therapeutics, current manufacturing systems for many glycosylated proteins require several months of development. Consequently, in the event of mass-casualty epidemics, current systems will fail to provide sufficient and timely quantities of emergency medical counter measures. As the identity of many new biological threats are unlikely to be known in advance, pre-emptive manufacturing and stockpiling of countermeasures cannot always be performed. Preparedness for all biological catastrophes requires a radical solution to replace the current slow scale-up and manufacture of certain lifesaving medical countermeasures. Current clinical and commercial manufacturing methods for glycosylated proteins use stable cell lines for protein expression, wherein the gene coding for the protein of interest is stably integrated into the host cell genome. The generation, identification, banking, testing, and scale-up of suitable stable clones generally takes many months. Because this development time is not compatible with emergency manufacturing, an alternative method for rapid production of medical counter measure antibodies is needed. One such potential technique is transient gene expression. Transient gene expression is a common approach for production of research-grade antibodies. It is frequently
used to generate milligram to gram quantities of material within two to three weeks of DNA sequence confirmation. In the past, transient systems have been considered for emergency production of large quantities of antibodies, but dismissed due to low titers, high cost of DNA, uncertain regulatory environment, and the lack of sufficient, available manufacturing capacity. Recent developments, however, have substantially enhanced the viability of such an approach. This article will explore these developments and investigate the use of transient gene expression for rapid production of antibody-based medical countermeasures.